KEY RESOURCES TABLE Humphries et al.
REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Chemicals, Peptides, and Recombinant Proteins | ||
Potassium chloride | Sigma-Aldrich | Cat #: P3911, CAS: 7447-40-7 |
L-glutamic acid monosodium salt hydrate | Sigma-Aldrich | Cat #: G5889, CAS: 142-47-2 (anhydrous) |
Glycerol | Sigma-Aldrich | Cat #: G5516, CAS: 56-81-5 |
Magnesium chloride hexahydrate | Fisher Scientific | Cat #: BP214, CAS: 7786-30-3 |
Potassium phosphate monobasic | Fisher Scientific | Cat #: BP362, CAS: 7778-77-0 |
Potassium phosphate dibasic | Fisher Scientific | Cat #: BP363, CAS: 7758-11-4 |
Thiamine HCl | Fisher Scientific | Cat #: BP892, CAS: 67-03-8 |
Manganese chloride | Acros Organics | Cat #: AC193451000, CAS: 13446-34-9 |
Calcium chloride | Fisher Scientific | Cat #: BP510, CAS: 10035-04-8 |
Iron (III) chloride | Acros Organics | Cat #: AC217090025, CAS10025-77-1 |
Zinc (II) chloride | Sigma-Aldrich | Cat #: Z0152, CAS: 7646-85-7 |
Thioflavin T | Acros Organics | Cat #: AC211761000, CAS: 2390-54-7 |
Isopropyl β-D-1-thiogalactopyranoside | Sigma-Aldrich | Cat #: IPTG-RO, CAS: 367-93-1 |
Asante Potassium Green (AM) | TEFLabs | Cat #: 3602 |
MOPS | Sigma-Aldrich | Cat #: M3183, CAS: 1132-61-2 |
Experimental Models: Organisms/Strains | ||
B. subtilis NCIB 3610 | Bacillus Genetic Stock Center | BGSCID: 3A1 |
B. subtilis NCIB 3610 AmyE::Phyp-mKate2, sinI::neo (motile cells) | This study | N/A |
B. subtilis NCIB 3610 AmyE::Phyp-YFP, hag::cat (non-motile cells) | This study | N/A |
B. subtilis NCIB 3610 trkA::neo (ΔtrkA) | Prindle et al., 2015) | N/A |
B. subtilis NCIB 3610 AmyE::Phyp-mKate2, ktrA::mls (ΔktrA) | This study | N/A |
P. aeruginosa PA01/pJA06-miniRK2 PSpac-tdTomato::kan | This study | N/A |
Software and Algorithms | ||
MATLAB and Image Processing Toolkit | MathWorks, Inc. 2012 | https://www.mathworks.com/products/matlab/ |
Python (Anaconda) | Continuum Analytics, 2016 | https://www.continuum.io/ |
Trackpy | Trackpy Contributors 2016 | http://soft-matter.github.io/trackpy/v0.3.2/# |
FIJI and Stitching Plugin | Schindelin, J et al. 2015, Preibisch et al. 2009 | https://fiji.sc/ |
Oufti | Paintdakhi et al. 2016 | http://oufti.org/ |
Other | ||
CellASIC Y04D microfluidic plates | EMD Millipore | Cat #: Y04D-02-5PK |
CellASIC ONIX microfluidic platform | EMD Millipore | Cat #: EV262 |