Skip to main content
. Author manuscript; available in PMC: 2018 Jan 12.
Published in final edited form as: Cell. 2017 Jan 12;168(1-2):200–209.e12. doi: 10.1016/j.cell.2016.12.014

KEY RESOURCES TABLE Humphries et al.

REAGENT or RESOURCE SOURCE IDENTIFIER
Chemicals, Peptides, and Recombinant Proteins
Potassium chloride Sigma-Aldrich Cat #: P3911, CAS: 7447-40-7
L-glutamic acid monosodium salt hydrate Sigma-Aldrich Cat #: G5889, CAS: 142-47-2 (anhydrous)
Glycerol Sigma-Aldrich Cat #: G5516, CAS: 56-81-5
Magnesium chloride hexahydrate Fisher Scientific Cat #: BP214, CAS: 7786-30-3
Potassium phosphate monobasic Fisher Scientific Cat #: BP362, CAS: 7778-77-0
Potassium phosphate dibasic Fisher Scientific Cat #: BP363, CAS: 7758-11-4
Thiamine HCl Fisher Scientific Cat #: BP892, CAS: 67-03-8
Manganese chloride Acros Organics Cat #: AC193451000, CAS: 13446-34-9
Calcium chloride Fisher Scientific Cat #: BP510, CAS: 10035-04-8
Iron (III) chloride Acros Organics Cat #: AC217090025, CAS10025-77-1
Zinc (II) chloride Sigma-Aldrich Cat #: Z0152, CAS: 7646-85-7
Thioflavin T Acros Organics Cat #: AC211761000, CAS: 2390-54-7
Isopropyl β-D-1-thiogalactopyranoside Sigma-Aldrich Cat #: IPTG-RO, CAS: 367-93-1
Asante Potassium Green (AM) TEFLabs Cat #: 3602
MOPS Sigma-Aldrich Cat #: M3183, CAS: 1132-61-2
Experimental Models: Organisms/Strains
B. subtilis NCIB 3610 Bacillus Genetic Stock Center BGSCID: 3A1
B. subtilis NCIB 3610 AmyE::Phyp-mKate2, sinI::neo (motile cells) This study N/A
B. subtilis NCIB 3610 AmyE::Phyp-YFP, hag::cat (non-motile cells) This study N/A
B. subtilis NCIB 3610 trkA::neo (ΔtrkA) Prindle et al., 2015) N/A
B. subtilis NCIB 3610 AmyE::Phyp-mKate2, ktrA::mls (ΔktrA) This study N/A
P. aeruginosa PA01/pJA06-miniRK2 PSpac-tdTomato::kan This study N/A
Software and Algorithms
MATLAB and Image Processing Toolkit MathWorks, Inc. 2012 https://www.mathworks.com/products/matlab/
Python (Anaconda) Continuum Analytics, 2016 https://www.continuum.io/
Trackpy Trackpy Contributors 2016 http://soft-matter.github.io/trackpy/v0.3.2/#
FIJI and Stitching Plugin Schindelin, J et al. 2015, Preibisch et al. 2009 https://fiji.sc/
Oufti Paintdakhi et al. 2016 http://oufti.org/
Other
CellASIC Y04D microfluidic plates EMD Millipore Cat #: Y04D-02-5PK
CellASIC ONIX microfluidic platform EMD Millipore Cat #: EV262
HHS Vulnerability Disclosure