Top: The mean number of labored breathing events during weekly methacholine (MCH) or vehicle exposure presented for the four treatment groups: Control (CON), Airway Inflammation (AI), Labored Breathing (LB), and Airway Inflammation + Labored Breathing (AI+LB). Mice that experienced MCH (LB and AI+LB) had significantly higher scores than those that did not experience MCH (CON and AI), indicated by *** (p<0.001). Bottom: MCH treatments also caused significantly higher mean enhanced pause (Penh) values compared to saline on P36, 43, 50, and 57, indicated by * (p<0.05).