Figure 10.

GlialCAM expression in the mouse and human brain throughout life. (A,C) Western blotting (A) and qPCR (C) in P0 to 12‐month‐old wild‐type mice show that GlialCAM protein (lower panel, approximately 60 kDa) and GlialCAM mRNA levels show the most pronounced increase up to P21 and then little further change. (B) Western blotting of frontal white matter lysates of normal human subjects shows that GlialCAM protein levels (lower panel, approximately 60 kDa) are highest during the first 5 years of life, decrease and stabilize thereafter. In‐gel trichloroethanol (TCE, A and B, upper panels) confirms equal protein load. (D) Real‐time qPCR analysis shows that relative GlialCAM mRNA levels are also highest in early infancy and then decrease to stabilize at around 5 years of life. Notably, the 13‐year‐old subject with highest GlialCAM mRNA levels had multiple injuries and succumbed after an unknown agonal time; no neuropathology was reported by the provider (National Institute of Child Health and Human Development Brain and Tissue Bank for Developmental Disorders at the University of Maryland, Baltimore, Maryland), but we cannot exclude presence of trauma‐related brain edema and compensatory GlialCAM overexpression.