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. Author manuscript; available in PMC: 2017 Aug 16.
Published in final edited form as: Anal Chem. 2016 Jul 27;88(16):8264–8271. doi: 10.1021/acs.analchem.6b02104

Figure 3.

Figure 3

Mechanism analysis of cell deflection. (a) The real parts of the CM factors of live PC-3 cells, dead PC-3 cells, and 20 μm Polystyrene beads calculated with the two-layer model. The conductivity of the surrounding medium is set as 14 mS/m and its relative permittivity is set as 80. The conductivity of cell membrane and cell cytoplasm, the relative permittivity of cell membrane and cell cytoplasm of live PC-3 cells are set as 1×10−4 mS/m, 1000 mS/m, 20, and 60, respectively. The conductivity and relative permittivity of the polystyrene beads are set as 0.05 mS/m and 60, respectively. The conductivity of cell membrane and cell cytoplasm, the relative permittivity of cell membrane and cell cytoplasm of dead PC-3 cells are set as 1×10−2 mS/m, 500 mS/m, 10, and 60, respectively. The diameter of the live and dead PC-3 cells is 16 μm. (b) The cells move out of the device without deflection, when modified DEP buffer is introduced from I1 (50 μL/hr) and original DEP buffer is introduced from I2 (250 μL/hr), and no electric field is applied. (c) The cells move out of the device with deflection, when modified DEP buffer is introduced from I1 (50 μL/hr) and original DEP buffer is introduced from I2 (250 μL/hr), and electric field is applied. Scale bar: 100 μm.