Figure 3.

Effect of glucose Ca²⁺ influx in A7r5 VSM cells. Effect of HG and NG on store-operated Ca²⁺ entry (SOCE) (a, b) and on protein expression of STIM and Orai1 (c-d). Cells were loaded with Fura2-AM and SOCE stimulated after store depletion with 1 μM thapsigargin (TG), an irreversible inhibitor of SERCA (a, b). Protein expression of STIM and Orai1 was determined between the different groups by western blot (c-d). Densitometry analysis was performed using Gene Tools, Geliance 600 Imaging system. Data are presented as mean ± SE from at least three independent experiments done each in triplicate. ^∗p < 0.05; NS: not significant.