Figure 1.
Characterization of MSCs isolated from human synovial membranes. (a) SM-MSCs from passage 3 exhibited a typical spindle-shaped morphology under an inverted microscope. (b) Representative colony-forming unit analysis. (c) Phenotypic analysis of SM-MSCs by flow cytometry. Histograms showed levels of surface antigen expression and their corresponding isotype control. (d) Multilineage differentiation potential of SM-MSCs. Samples were stained with Oil Red O, indicating differentiated adipocytes, and with Alizarin Red S staining, indicating mature osteoblasts. Original magnification, ×100. SM-MSCs, synovial membrane-derived MSCs.