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. Author manuscript; available in PMC: 2017 Jul 5.
Published in final edited form as: Appl Microbiol Biotechnol. 2015 Jun 9;99(20):8667–8680. doi: 10.1007/s00253-015-6708-9

Fig. 6.

Fig. 6

Effect of PEP3 overexpression on V-ATPase activity. a S288c leu2Δ/pGP564-PEP3 (black bar) exhibited better relative growth in the presence of the V-ATPase inhibitor concanamycin A than S288c leu2Δ/pGP564 (white bar) in YEPG-5 and in b YEPD-7.5. Data are means± standard deviations (n=3). Asterisks indicate a significant difference between the strains, **p<0.01, ***p<0.001. The relative growth assay is defined in “Materials and methods” (indirect measurement of V-ATPase activity)