Fig. 3.
Addition of FGF-2 does not affect the levels of let-7 family members. (A) MSCs were cultured for 2 days in the presence FGF-2 (10 ng/ml). Then, total RNA was extracted and miRNA levels were measured by RT-PCR (N = 4). (B) Similarly, MSCs were transduced to over-express LIN28, and after 3–4 days, miRNA levels were measured. LIN28 over-expression specifically reduces the let-7 family members but has no effect on other miRNA (N = 4). *P <0.05, **P <0.005, and ***P <0.0005.