Figure 6. OxySite preserves glucose stimulated insulin release for rat pancreatic islets cultured under hypoxic conditions.
A–B) Dynamic glucose perifusion and resulting insulin release from islets cultured under low oxygen (0.01 mM) without (orange) or with OxySite (blue) after acute (8 h; A) and chronic (24 h; B) culture. Grey region = high glucose (11 mM); Striped region = KCl C) Resulting area under the curve (AUC) of insulin release during high glucose stimulation for low oxygen cultures without (white bar) or with OxySite (black bar), compared to islet cultured under standard oxygen and density conditions (free islets; grey bar). D) KCL Stimulation Index (SI) for low oxygen cultures without (white bar) or with OxySite (black bar). * p < 0.05,** p < 0.01.