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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: Biomaterials. 2017 Mar 18;129:139–151. doi: 10.1016/j.biomaterials.2017.03.018

Figure 9. Co-culture of rat islets with OxySite resulted in enhanced functional outcomes when transplanted into diabetic syngeneic recipients.

Figure 9

A) Average nonfasting blood glucose levels of rats receiving islets cultured under low oxygen (0.01 mM) conditions with OxySite for 24 h prior to transplant (black squares; n = 8). Control islets were cultured under standard oxygen (0.2 mM) for 24 h prior to transplant (grey circles; n = 8) or with a blank PDMS (grey line; n=8). B) Tracking of glucose clearance following intravenous glucose tolerance test (IVTT) performed in a subset of animals at 30 d post-transplant. Metabolic clearance was compared to a nondiabetic rat. (OxySite n = 5; Control, n = 3; Nondiabetic control, n = 1). D) Immunohistochemistry evaluation of grafts explanted 40 days post-transplant, stained with anti-insulin (green), anti-smooth muscle actin (SMA, red), and nuclear staining (blue) (top row) or anti-insulin (green), anti-glucagon (pink), and nuclear staining (blue) (top row). Scale bar = 50 μm.