Figure 1.

Catecholamines upregulate Sirt1 expression at both mRNA and protein levels. (a–d) SiHa and CaSki cells were starved overnight and then treated with 10 μM ISO or 1 μM EPI. The cells were harvested at the indicated time points. The expressions of Sirt1 and c‐Myc in the cell lysates were analyzed by Western blot. (e, f) SiHa and CaSki cells were treated with 0, 0.625, 1.25, 2.5, 5, or 10 μM ISO for 3 h. The expressions of Sirt1 and c‐Myc were analyzed by Western blot. (g, h) The total RNA was isolated from SiHa and CaSki cells that were treated with 10 μM ISO. The expression of the Sirt1 mRNA was assessed by real‐time RT‐PCR. (i) CaSki cells were transfected with the specific siRNA targeting c‐Myc or control siRNA. After transfection for 24 h, the cells were treated with ISO, EPI, or vehicle for 3 h. The expression of c‐Myc was analyzed by Western blot. (j) CaSki and SiHa cells were transfected with the specific siRNA targeting c‐Myc or control siRNA. After transfection for 24 h, the cells were treated with ISO, EPI, or vehicle for 3 h. The expression of Sirt1 was analyzed. **P < 0.01