Figure 5.

Catecholamines induce resistance of cervical cancer cells to chemotherapeutics in vitro and in vivo. (a–d) SiHa cells were cotreated with 2.5 μM ISO or 1 μM EPI and DOX or DDP. The cell proliferation activities were determined by CCK8 assays. (e) SiHa cells were treated with 0.5 μM DOX or 2.5 μM ISO. After treatment for 24, 36 or 48 h, the cell proliferative activities were determined by CCK8 assays. (f, g) Five to 6‐week‐old athymic female Balb/C nude mice received PBS or ISO (10 mg/kg) by intraperitoneal injections, commencing 2 day before inoculation of tumor cells. A total of 0.1 mL SiHa cell suspension (5 × 10⁷ cells/mL) was injected subcutaneously in the upper right flank of the mice. DOX treatment (10 mg/kg) was started 5 day after implantation of SiHa cells. At the end of the experiments (4 weeks), the mice were sacrificed and the primary tumors were dissected, weighted, and photographed. **P < 0.01