Fig. 11. Fluorescence microscopy images of live HeLa cells incubated with 4 μM CuQLT at 37 °C for 15 min in PBS. Top: control cells. (A) DIC image. CuQLT signal (B) before and (C) 15 min after treatment with 1.5 mM GSNO on the microscope stage. Bottom: cells pre-incubated with 1 mM N-methylmaleimide (NMM) in PBS for 30 min at 37 °C. (D) DIC image. CuQLT signal (E) before and (F) 15 min after treatment with 1.5 mM GSNO on the microscope stage. (G) Quantification of the intracellular fluorescence response of CuQLT (mean ± SD, N = 21 for control cells, N = 20 for NMM-treated cells). Scale bar = 15 μm. For full-field images and associated nuclear staining, see Fig. S33.† .
