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Fig. 8. Co-localization analysis of CuCLT with organelle-specific markers in live HeLa cells incubated with 4 μM CuCLT, 15 μM Hoechst 33258, and 100 nM LysoTracker Green at 37 °C for 15 min in dye- and serum-free DMEM. (A) DIC image. (B) Signal from Hoechst 33258. (C) Signal from LysoTracker Green. (D) Signal from CuCLT. (E) Overlay of Hoechst 33258 and CuCLT. (F) Overlay of LysoTracker Green and CuCLT. Scale bar = 15 μm.