Fig. 4. Insertions of roX1 and 1.688^3F on 2L partially rescue males with a lethal 2L deficiency.

A) Df(2)22A2 is lethal in males (top left) and females (top right). If a transgene on the homolog recruits compensation, male-limited rescue will occur (bottom left). Thick lines indicate compensated chromatin. Females block formation of the MSL complex, preventing rescue (bottom right). B) Pharate Df(2)22A2 males are observed only when [roX1]^22A3, [1.688^3F]^22A3 or [roX1+1.688^3F]^22A3 is present on the homolog. The recovery of pharate males is calculated from brothers that emerged with an intact 2^nd chromosome (See Fig. S6A for mating strategy). C) Recovery of Df(2)22A2 female larvae is unaffected by [roX1+1.688^3F]^22A3 on the homolog (See Fig. S7B for mating strategy). D) Recovery of Df(2)22A2 male larvae increases when [roX1]^22A3, [1.688^3F]^22A3 or [roX1+1.688^3F]^22A3 is present on the intact homolog (hatched bars), but not when a control P{EPgy2}^hafEY08668 transgene marked with y⁺ and w⁺ is inserted at 22A3 (Control TG). Expression of siRNA to white (gray) has no effect on recovery of male larvae, but expression of 1.688^3F siRNA (black) enhances recovery when transgenes containing 1.688^3F DNA are present on the intact homolog (mating strategy in Fig. S7A, C). See Figure S1 for transgene composition and Figure S4 for 1.688^3F sequence. Error bars represent SEM. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.