Fig. 5. Insertion of 1.688^1A and 1.688^3C on 2L partially rescue males with a lethal 2L deficiency.

Recovery of Df(2)22A2 male larvae increases when [1.688^1A]^22A3, [roX1+1.688^1A]^22A3, [1.688^3C]^22A3 or [roX1+1.688^3C]^22A3 is present on the intact homolog (hatched bars). The ratio of male larvae carrying Df(2)22A2 to brothers with an intact 2^nd chromosome is presented. Recovery of males with no transgene or [roX1]^22A3 is redrawn from Fig. 4D. Ectopic expression of 1.688^3F siRNA (black) enhances recovery when 1.688^1A or 1.688^3C DNA is present on the homolog. The mating strategy is presented in Figure S7A, C. See Figure S1 for transgene composition and Figure S4 for comparison of 1.688^X sequences used. Error bars represent SEM. ** p < 0.01; *** p < 0.001.