Table 1. Autosomal MSL2 recruitment by [roX1]22A3; [1.6883F]22A3 and [roX1+1.6883F]22A3.
Recruitment close to the integration site is ranked from none (-) to very strong (++++). Additional autosomal signals are categorized by cytological position. The percentage of nuclei in each category is followed by the number of nuclei (parentheses). Counts were derived from 3 individuals with no transgene (yw laboratory reference strain) and groups of 5 individuals with [roX1]22A3, [1.6883F]22A3 or [roX1+1.6883F]22A3. Scoring was done on coded samples to prevent bias. Representative images depicting weak (+) to very strong (++++) recruitment are presented in Figure S2A.
| Transgene | |||||
|---|---|---|---|---|---|
| None | roX1 | 1.6883F | roX1 + 1.6883F | ||
| 22A3 Recruitment | - | 100 (63) | 0 (0) | 0 (0) | 1.01 (1) |
| + | 0 (0) | 10.78 (11) | 0 (0) | 0 (0) | |
| ++ | 0 (0) | 65.68 (67) | 15.17 (17) | 30.3 (30) | |
| +++ | 0 (0) | 23.52 (24) | 55.35 (62) | 54.54 (54) | |
| ++++ | 0 (0) | 0 (0) | 29.46 (33) | 14.14 (14) | |
| Additional signal on 2L | None | 98.41 (62) | 75.49 (77) | 9.82 (11) | 15.15 (15) |
| Sub-telomeric | 0 (0) | 6.86 (7) | 89.3 (100) | 78.78 (78) | |
| Proximal to 22A3 | 1.5 (1) | 19.6 (20) | 8.03 (9) | 4.04 (4) | |
| 3L telomere | None | 98.41 (62) | 95.09 (97) | 49.1 (55) | 45.45 (45) |
| One Band | 1.58 (1) | 4.90 (5) | 50.89 (57) | 54.54 (54) | |
| Other | Ectopic bands on other chr | 3.17 (2) | 36.27 (37) | 7.14 (8) | 9.09 (9) |
| X-staining normal | 100 (63) | 100 (102) | 100 (112) | 100 (99) | |
| Total nuclei counted | 63 | 102 | 112 | 99 | |