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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Placenta. 2017 Apr 12;55:29–36. doi: 10.1016/j.placenta.2017.04.006

Fig 3. Protein and cholesterol content of density gradient fractions from BeWo cells following ultracentrifugation.

Fig 3

Plasma membranes collected from BeWo cells treated with HBSS, MβCD (5 mM, 1 h), or MβCD (5 mM, 1 h) and then MβCD-cholesterol (5 mM, 30 min) were subjected to density gradient ultracentrifugation in order to isolate detergent-resistant lipid rafts. Ten 1.4 mL fractions were collected and analyzed for protein or cholesterol content as described in the Materials and Methods. Data are presented as mean ± SE (n = 3 independent experiments).