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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Placenta. 2017 Apr 12;55:29–36. doi: 10.1016/j.placenta.2017.04.006

Fig 4. Localization of the BCRP transporter following cholesterol depletion of BeWo cells.

Fig 4

Western blots were performed using membrane fractions collected from BeWo cells following treatment with vehicle (Control), 5 mM methyl-β-cyclodextrin (MβCD) for 1 h, or 5 mM MβCD for 1 h and then 5 mM cholesterol-loaded MβCD for 30 min (MβCD + CHOL). Western blots show BCRP along with lipid raft (PLAP) and non-lipid raft (TFR) markers in fractions obtained from 1 representative experiment. Enrichment of proteins in different fractions was semi-quantified using densitometry of Western blots from 3 independent experiments. Data are presented as mean ± SE *p < 0.05 compared to control within each fraction.