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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Placenta. 2017 Apr 12;55:29–36. doi: 10.1016/j.placenta.2017.04.006

Fig 5. Relationship between cholesterol content and BCRP substrate retention in BeWo cells.

Fig 5

BeWo cells were treated with vehicle or pravastatin (10 – 200 μM) for 48 h. A. Effects of pravastatin on cholesterol content. Cholesterol was measured in cell lysates using the Amplex Red assay. B. Effects of pravastatin on BCRP efflux. Efflux transporter activity was indirectly measured by the ability of cells to retain the BCRP substrate Hoechst 33342. The BCRP substrate was quantified in relative fluorescence units (RFU) using a Nexcelom Cellometer (n = 3 independent experiments).*p<0.05 relative to control. Data are presented as mean ± SE.