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. Author manuscript; available in PMC: 2018 May 8.
Published in final edited form as: Dev Cell. 2017 May 8;41(3):262–273.e6. doi: 10.1016/j.devcel.2017.04.003

Fig. 5. ML1 promotes polarized TVs trafficking towards apical membranes.

Fig. 5

(A) Patch-clamp recording from basolateral (standard whole-cell recording) or apical (lyse cell to expose VACs before whole-VAC recording) membranes. Note that the extracellular side of the apical membrane is facing the VAC lumen. (B, C) Representative whole-VAC (apical membrane) recordings of ML1-like currents in histamine-stimulated WT (B) and ML1PC (C) parietal cells. (D, E) Representative whole-cell (basolateral membrane) detection of IML1 in WT and ML1PC cells. (F) Group averages of data from experiments shown in panels B–E (n = 3–5 patches/condition). Bar graphs show means ± SEM from ≥3 experiments. *P < 0.05, **P < 0.01, ***P < 0.001 one-way ANOVA, Bonferroni’s post-hoc analysis.