Skip to main content
. 2017 Jun 14;108(7):1328–1337. doi: 10.1111/cas.13277

Figure 3.

Figure 3

HBx inhibited ARID2 mRNA expression at the transcriptional level. (a) Luciferase activity of the human ARID2 promoter construct pGL3‐ARID2 in Huh7 cells. Huh7 cells were transfected with pGL3‐ARID2 (position ‐1040 to +101) for 24 h and then infected with AdGFP control or AdHBx. At 36 h postinfection, cells were harvested for luciferase assays. Data are presented as the mean (±SD) relative luciferase activity compared with the activity of the pGL3‐Basic control sample. Three independent experiments were performed. *P < 0.05; **P < 0.01 by Student's t‐test. (b) Luciferase assays of human ARID2 promoter constructs with the wild‐type sequence (‐1040/+101 nt) or the indicated serial deletion mutations in AdHBx‐ or AdGFP‐infected Huh7 cells (n = 3, *P < 0.05). (c) ChIP assays of cell extracts from Huh7 cells infected with AdHBx or AdGFP. Huh7 cells were infected with AdHBx or AdGFP control. At 48 h post‐infection, cell lysates were collected, and ChIP analysis was performed using control IgG or anti‐HBx antibodies. Transcriptional factor E2F1 recruited on ARID2 and CCND1 promoter were used as ChIP positive control.