Figure 3.

Knockdown of acetyl‐CoA synthase 2 (ACSS2) promoted epithelial–mesenchymal transition of hepatocellular carcinoma cells without increasing hypoxia‐inducible factor‐2α (HIF‐2α). (a) Morphology of MHCC97H after siRNA interference in normoxic and hypoxic conditions. (b) Western blot images show that there were more N‐cadherin, vimentin, and Snail, and less E‐cadherin expression in the siACSS2 cells compared to the normal control (NC) cells in hypoxic conditions. Grayscale analysis of N‐cadherin and E‐cadherin is shown as histograms, which represent three replicates. Data in the histograms are normalized to β‐actin. (c) There was no difference in HIF‐2α expression between the siACSS2 group and the NC group. Grayscale analysis of HIF‐2α is also shown. Error bars represent SD. *P < 0.05; **P < 0.01.