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. 2017 Jul 4;108(7):1357–1367. doi: 10.1111/cas.13262

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© 2017 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Cancer stem cells of human colorectal carcinomas (CoCSC) generate endothelial cells (EC) expressing the endothelial antigen VEGFR2 and CD144 in xenograft tumors. (a) Representative immunofluorescence micrographs of tumor xenograft sections labeled with VEGFR2 (i) and NuMA (ii) showed that some EC expressed the markers of EC and NuMA. (b) Tripe staining with CD31 (i), VEGFR2 (ii) and NuMA (iii) show that some EC co‐expressing CD31 and VEGFR2 are NuMA ⁺. Arrowheads indicate NuMA ⁺ EC and arrows indicate NuMA ⁻ EC. (c) Representative immunofluorescence micrographs of tumor xenograft sections labeled with CD144 (i) and NuMA (ii) showed that some EC expressed the markers of EC and NuMA. (d) IF staining of CD144 and NuMA showed that some tumor cells expressed CD144. Arrowheads indicate NuMA ⁺ EC. Scale bars: 20 μm.