Figure 5.

Blood flow in the vessels of cancer stem cells of human colorectal carcinomas (CoCSC) xenograft. Lectin which can bind to endothelial cells (EC) was injected into the tail veins of the mice. Representative immunofluorescence micrographs showed that a vessel constituted by NuMA ⁻ endothelial cells (EC) (a) or RFP ⁻ EC (d), a vessel constituted by RFP ⁺ EC (c) and a vessel containing several NuMA ⁺ EC (b) were functional. (e) Nonfunctional RFP ⁺ EC‐forming vessels were also observed. (f) Nonfunctional RFP ⁻ vessels were also observed. Arrowheads indicate NuMA ⁺ or RFP ⁺ EC and arrows indicate NuMA ⁻ or RFP ⁻ EC. Scale bars: 20 μm.