Table 1.
Antibodies used for immunohistochemistry in the present study
| Molecule | Antibody | Dilution | Localization | Control tissue | ||
|---|---|---|---|---|---|---|
| Type | Clone/code | Manufacturer | ||||
| Estrogen receptor | Mono (M) | 1D5 | Dako | ×100 | N | Mammary gland |
| Progesterone receptor | Mono (M) | PgR636 | Dako | ×100 | N | Mammary gland |
| HER2 | Poly (R) | HercepTest | Dako | Ready to use | Me | Breast cancer |
| CK5/6 | Mono (M) | D5/16 B4 | Dako | ×100 | C | Mammary gland |
| EGFR | Poly (R) | PharmDx | Dako | Ready to use | C, Me | Epidermis |
| BRCA1 | Mono (M) | MS110 | Calbiochem | ×100 | N | Mammary gland |
| ZEB1 | Poly (R) | ab87280 | Abcam | ×100 | N | MCF7, MDA‐MB‐231 |
| TWISTNB | Poly (R) | PAB21491 | Abnova | ×50 | N | Cerebellum |
| Vimentin | Mono (M) | V9 | Dako | ×100 | C | Fibroblast |
| E‐cadherin | Mono (M) | NCH‐38 | Dako | ×100 | Me | Epidermis |
| Snail‐2 | Poly (R) | PAB1923 | Abnova | ×200 | N | MCF7, MDA‐MB‐231 |
| HMGB1 | Mono (M) | 2F6 | Abnova | ×100 | C, N | Placenta |
| Androgen receptor | Mono (M) | AR441 | Dako | ×50 | N | Mammary gland |
C, cytoplasm; M, mouse; Me, membrane; N, nucleus; R, rabbit.