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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: Anal Biochem. 2017 Mar 27;526:66–68. doi: 10.1016/j.ab.2017.03.019

Fig. 2. Nicked linear pieces are additionally fragmented during electrophoresis.

Fig. 2

A. A scheme of the experiment: early-stationary cells were exposed to UV and made into agarose plugs as described in figure 1. The agarose plugs were washed in TE, digested with Not I and subjected to pulsed-gel electrophoresis to reveal any decrease in 1 Mbp band [11].

B. A representative pulsed-field gel of NER+ (AB1157) versus NER− (uvrA mutant, SRK303-1) cells. 1 Mb, the 1 Mbp Not I fragment, whose quantity is shown in “C” as the fraction of the total DNA signal in the gel.

C. Quantification of several gels like in “B”. The data are averages ± SEM, with n = 4 or 5. P is two-tail probability that the two values are different. The two values in the NER+ cells are (P<<0.05).