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. 2017 Jun 25;108(7):1405–1413. doi: 10.1111/cas.13276

Figure 5.

Figure 5

Abolishing the regulation of RIP1 K63‐linked ubiquitination by p62/SQSTM1 increases the sensitivity of SKOV3 cells to cisplatin. (a) The viability of cells was analyzed by an MTT assay. Data were presented as mean ± SD, n = 3. *P < 0.05, vs wt‐p62. (b) SKOV3 cells were treated as before. Cells were stained with Trypan blue and observed under a microscope. Data were presented as mean ± SD, n = 3. *P < 0.05, vs wt‐p62. (c) The apoptosis ratio of SKOV3 cells was detected by flow cytometry analysis. (d) Cells were analyzed using a caspase3/7 activity assay (Promega). Data are presented as mean ± SD, n = 3. *P < 0.05 vs wt‐p62. (e) The mRNA expression of BCL2L1 in SKOV3 cells after transfection of wt‐p62 and ΔZZ‐p62 plasmid. ACTB was used for normalization.Data are presented as mean ± SD, n = 3. *P < 0.05 vs wt‐p62.