Fig 3. Induction and recovery times during immersion anaesthesia with metacaine of large fish (600–1300 g).

Induction and recovery times with different doses of (A) metacaine at 6°C, (B) metacaine at 12°C. The colored boxes show times for induction and recovery. Average time in minutes ± S.D. is shown. Red boxes = induction times, black boxes = initial respiration, blue boxes = normal respiration, grey boxes = swimming activity. Single letters means that this measurement is statistically different the other. Shared letters means that the differences are not statistically significant (P < 0.05). N = 5. Statistical analyses for induction and each of the phases during recovery, as well as statistical analyses of the effect of temperature, are given in S4–S7 Tables.