. 2017 Jul 5;12(7):e0179344. doi: 10.1371/journal.pone.0179344

Table 1. Overview of behaviour of lumpfish at different stages of anaesthesia ¹.

Stage	Plane	Description	Appearance	Swimming activity	Equilibrium	Responsiveness²	Muscle tone	Respiration
0		Normal	Normal	Normal/adhere to the substrate	Normal	Yes	Normal	Normal
I		Light sedation	Disoriented	Reduced	Normal/ reduced	Reduced	Normal	Normal
II^*		Excitatory	Excited	Increased	Struggles to maintain balance	n.d.	Normal	Irregular/ increased
III	1	Light anaesthesia	Anaesthetized	Stopped	Lost	None	Decreased	Normal or decreased
	2	Surgical anaesthesia	Anaesthetized	Stopped	Lost	None	Relax	Shallow
	3	Deep narcosis	Anaesthetized	Stopped	Lost	None	None	Nearly absent
IV		Impending death	Moribund	Stopped	Lost	None	None	Stopped

¹ The Table has been modified from Zahl et al. [32]

² Responsiveness refers to reaction to external stimuli (pinch in the lower lip)

*Observed at high concentrations of metacaine (> 400 mg L^-1)

Table 1. Overview of behaviour of lumpfish at different stages of anaesthesia 1.