Fig 3. Pharmacokinetic/pharmacodynamic studies.

Maximal changes in mean arterial blood pressure (A) and detection of (peak) exhaled H₂S gas (in parts per million [ppm]) (B) following increasing IV bolus doses of ammonium tetrathiomolybdate (ATTM) or NaHS. No exhaled H₂S was detectable following ATTM administration. Acid/base interactions following ATTM treatment are shown in (C); the top left y-axis denotes (arterial) partial pressure of carbon dioxide (PCO₂); bottom left and right y-axes are (arterial) base excess and pH, respectively. Alterations in (arterial) glucose and lactate following ATTM treatment are shown in (D). (E) shows the absorbance (ultra violet—visible) spectrum of ATTM with (inset) a row of microplate wells used to construct a standard curve. (F) and (G) respectively show changes in ATTM plasma levels (measured using the absorbance peak at 468 nm at 2 min after ATTM administration) against the quantity of drug administered and subsequent (25 min later) changes in arterial pH. n = 3–4/group.