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. 2017 Jul 5;12(7):e0179783. doi: 10.1371/journal.pone.0179783

Fig 9. miR-182 down-regulated the expression level of PTN via the 3′ UTR.

Fig 9

(A) Schematic diagram illustrating the design of luciferase reporters with the WT- PTN 3′ UTR (WT-PTN) or the site-directed mutant PTN 3′ UTR (Mut-PTN). The nucleotides in red represented “seed sequence” of miR-182, the mutation nucleotides were in yellow color. (B) psiCHECKTM-2 vector map and the insertion site of PTN-3′UTR marked in green color. (C) The PTN -3′ UTR or its mutation luciferase reporter vectors were co-transfected with miR-182 mimic (or negative control) into 293T cells. Luciferase assay was performed 24 h after transfection. Results are represented as relative luciferase activity. The results are represented as mean ± SD (n = 3×3); *p < 0.05, ** p < 0.01.