Table 1. The RT-qPCR Primers used in the present study.
| gene | GenBank accession No. |
Primer sequences (5′→3′) |
|
|---|---|---|---|
| GADPH | AF_030943.1 | F: GCAAGTTCCACGGCACAG | |
| R: GGTTCACGCCCATCACAA | |||
| PTN | XM_005679509.1 |
F: TCTCCATTTCCCTTCCTTCC R: TCTCTCTCCACTTCGGCTTT |
|
| PTN | XM_005679509.1 |
F: GCCTCGAGGACCGTGAAAAGGACATC R: GCGCGGCCGCCAGCATCACCTTGATTTA |
|
| 18S | / |
F: GTGGTGTTGAGGAAAGCAGACA R: TGATCACACGTTCCACCTCATC |
|
| U6 | / |
F: CTCGCTTCGGCAGCACA R: AACGCTTCACGAATTTGCGT |
|
| miR-182-Loop | / | gtcgtatccagtgcagggtccgaggtattcgcactggatacgacAGTGTGAG | |
| miR-182-FW | / | ggTGAAAAGTTCGTTCGG | |
| Reverse Primer | / | GTGCAGGGTCCGAGGT | |
Note: the underscore characters were restriction enzyme cutting site of xho І and not І.