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. 2017 Jul 5;12(7):e0180478. doi: 10.1371/journal.pone.0180478

Fig 2. Phenotype of the psy2l knockout line (SALK_048064).

Fig 2

A, Three weeks old homozygous psy2l (arrows), verified by genotyping, had a distinct appearance as compared with WT (the two bigger plants). B-C, Ten weeks old psy2l showed dwarfism and late flowering. D, Anthocyanin accumulation in four weeks old psy2l while WT leaves to the right do not accumulate anthocyanins. E, Eight months old semi-sterile psy2l. Most of the siliques contained either few or no seeds. F-G, Alexander staining of viable pollen. In wild type, clearly all pollen was stained. In psy2l most of the pollen was stained, also some non-stained pollen was seen. H, I, Eight and 20 days old seedlings showing severe retardation in root and shoot development in psy2l compared with WT. J-M, Images of roots from 10–12 days old WT and psy2l plants showing abnormal roots for psy2l with extensive root hairs close to the root tip and disorganized root cells. In A-G and H-M, plants were grown under 12 h light/ 12 h dark and 16 h light/ 8 h dark conditions, respectively.