Skip to main content
. 2017 Jul 5;12(7):e0180478. doi: 10.1371/journal.pone.0180478

Fig 5. Subcellular targeting analysis for PP4 catalytic and regulatory subunits in onion epidermal cells.

Fig 5

Fusion proteins were precipitated on gold, bombarded into onion epidermal cells, and examined after 16 h. A, PSY2L with free N-terminus targeted only nucleus. B, PSY2L with free C-terminus targeted nucleus and cytosol. C-E, PP4R2L targeted cytosol (C and D), nucleus (C-E) and endoplasmic reticulum (D, E). Partial overlap between OFP-ER and free C-terminus PP4R2L was detected in (E). F-H, PP4.1 showed a variability of targeting patterns including cytosol (F-H) and weak nucleus targeting (F, H) and unknown punctate structures (F). In addition, in some cells, also targeting of the nuclear envelope was seen (G, H). I-K, PP4.2 protein showed mostly targeting to cytosol, and unknown punctate structures (I-K). Endoplasmic reticulum was labeled by OFP-ER [25]. Scale bars = 20 μM.