Table 2. mRNA expression in PBMC’s of melioidosis patients (n = 30) compared to other sepsis cases (n = 10) and healthy negative controls (n = 10).
| Melioidosis vs Healthy controls | Melioidosis vs Sepsis controls | Sepsis cases vs Healthy controls | ||||
|---|---|---|---|---|---|---|
| Gene | Relative expression ratio [95% CI] | P value | Relative expression ratio [95% CI] | P value | Relative expression ratio [95% CI] | P value |
| HMGB1 | 0.83[0.444,1.539] | 0.5324 | 0.26[0.132,0.510]* | 0.0005 | 3.18[1.539,6.578]* | 0.0036 |
| IL6 | 1.56[0.670,3.623] | 0.2912 | 0.89[0.360,2.189] | 0.7867 | 1.76[0.710,4.344] | 0.2079 |
| IL8 | 1.65[0.727,3.733] | 0.2187 | 0.43[0.169,1.080] | 0.0699 | 3.86[1.411,10.540]* | 0.0114 |
| IL1β | 1.33[0.554,3.205] | 0.5005 | 0.77[0.258,2.320] | 0.6254 | 1.72[0.516,5.738] | 0.3545 |
| IFNγ | 1.34[0.826,2.189] | 0.2174 | 0.88[0.496,1.572] | 0.6522 | 1.52[0.800,2.899] | 0.1864 |
| TNFα | 1.22[0.705,2.124] | 0.4573 | 0.81[0.408,1.598] | 0.5173 | 1.52[0.741,3.100] | 0.2365 |
| IL15 | 1.31[0.751,2.296] | 0.3254 | 0.57[0.258,1.279] | 0.1610 | 2.29[1.025,5.103]* | 0.0443 |
| IL4 | 1.78[0.115,27.531] | 0.3533 | 4.09[1.178,14.173]* | 0.0366 | 0.44[0.065,2.942] | 0.2329 |
| TLR2 | 1.16[0.666,2.034] | 0.5844 | 0.44[0.196,1.009] | 0.0522 | 2.62[1.182,5.803]* | 0.0212 |
| TLR4 | 0.97[0.532,1.777] | 0.9247 | 0.37[0.141,0.974]* | 0.0448 | 2.62[1.025,6.700]* | 0.0450 |
| MICB | 0.95[0.533,1.699] | 0.8607 | 0.33[0.183,0.582]* | 0.0006 | 2.92[1.534,5.545]* | 0.0026 |
| HLADMB | 0.84[0.502,1.396] | 0.4814 | 0.69[0.396,1.204] | 0.1809 | 1.21[0.682,2.156] | 0.4897 |
| PSMB2 | 0.88[0.513,1.519] | 0.6403 | 0.25[0.121,0.508]* | 0.0008 | 3.56[1.708,7.420]* | 0.0021 |
| PSME2 | 1.24[0.763,1.998] | 0.3757 | 0.38[0.195,0.726]* | 0.0061 | 3.28[1.684,6.389]* | 0.0017 |
| PSMB8 | 0.83[0.456,1.519] | 0.5356 | 0.27[0.131,0.565]* | 0.0014 | 3.06[1.443,6.480]* | 0.0060 |
| PSMA5 | 0.75[0.421,1.328] | 0.3086 | 0.84[0.412,1.706] | 0.6102 | 0.89[0.448,1.778] | 0.7301 |
| DNMT1A | 0.66[0.376,1.171] | 0.1494 | 0.51[0.211,1.213] | 0.1171 | 1.31[0.542,3.169] | 0.5224 |
| DNMT3A | 0.68[0.416,1.097] | 0.1087 | 0.68[0.356,1.300] | 0.2284 | 0.99[0.533,1.849] | 0.9793 |
| DNMT3B | 1.07[0.636,1.814] | 0.7770 | 0.22[0.088,0.539]* | 0.0040 | 4.94[1.948,12.503]* | 0.0031 |
| HDAC1 | 0.78[0.556,1.095] | 0.1441 | 0.50[0.353,0.719]* | 0.0006 | 1.55[1.059,2.267]* | 0.0266 |
| HDAC2 | 0.68[0.377,1.212] | 0.1799 | 0.28[0.126,0.642]* | 0.0048 | 2.38[1.047,5.403]* | 0.0398 |
| HDAC4 | 0.99[0.588,1.652] | 0.9549 | 0.64[0.322,1.270] | 0.1880 | 1.54[0.786,3.025] | 0.1911 |
PBMC, peripheral blood mononuclear cells; CI, confidence interval; Relative expression ratio based on efficiency corrected delta delta Ct values presented as log values to the base 2, >1.5 indicates up regulation and ≤0.5 indicates down regulation in the experimental group compared to control group
* indicates statistically significant differential expression where P-value <0.05, P values calculated by paired t-tests