Table 4. mRNA expression in PBMC’s of melioidosis patients with >15 days of fever (n = 25), melioidosis patients with ≤15 days of antibiotics (n = 15), melioidosis patients with >15 days of antibiotics (n = 12) compared to other sepsis cases (n = 10).
| Melioidosis (>15 days fever) vs Sepsis controls | Melioidosis (≤15 days of antibiotics) vs Sepsis controls | Melioidosis (>15 days of antibiotics) vs Sepsis controls | ||||
|---|---|---|---|---|---|---|
| Gene | Relative expression ratio [95% CI] | P value | Relative expression ratio [95% CI] | P value | Relative expression ratio [95% CI] | P value |
| HMGB1 | 0.29[0.149,0.566] * | 0.001 | 0.37[0.193,0.712]* | 0.0053 | 0.18[0.065,0.493]* | 0.0023 |
| IL6 | 1.10[0.444,2.732] | 0.8281 | 1.08[0.345,3.379] | 0.8895 | 0.88[0.244,3.140] | 0.8283 |
| IL8 | 0.53[0.206,1.345] | 0.1682 | 0.49[0.169,1.446] | 0.1869 | 0.44[0.142,1.366] | 0.1463 |
| IL1β | 0.94[0.310,2.851] | 0.9071 | 0.98[0.299,3.221] | 0.9744 | 0.71[0.202,2.467] | 0.5660 |
| IFNγ | 0.91[0.507,1.630] | 0.7333 | 0.97[0.518,1.805] | 0.9116 | 0.72[0.375,1.396] | 0.3133 |
| TNFα | 0.94[0.475,1.871] | 0.8585 | 0.90[0.411,1.987] | 0.7914 | 0.75[0.342,1.643] | 0.4508 |
| IL15 | 0.62[0.281,1.385] | 0.2263 | 0.55[0.234,1.311] | 0.1663 | 0.71[0.226,2.218] | 0.5196 |
| IL4 | 4.60[1.317,16.085]* | 0.0299 | 3.95[1.132,13.800]* | 0.0384 | - | |
| TLR2 | 0.51[0.229,1.144] | 0.0966 | 0.53[0.232,1.195] | 0.1167 | 0.37[0.105,1.317] | 0.1165 |
| TLR4 | 0.44[0.171,1.144] | 0.087 | 0.42[0.160,1.114] | 0.0776 | 0.32[0.078,1.328] | 0.1095 |
| MICB | 0.36[0.204,0.634]* | 0.0012 | 0.39[0.221,0.687]* | 0.0026 | 0.27[0.102,0.704]* | 0.0108 |
| HLADMB | 0.79[0.464,1.347] | 0.3671 | 0.84[0.483,1.444] | 0.4997 | 0.58[0.234,1.446] | 0.2246 |
| PSMB2 | 0.28[0.139,0.571]* | 0.0016 | 0.30[0.148,0.626]* | 0.003 | 0.21[0.078,0.552]* | 0.0032 |
| PSME2 | 0.43[0.225,0.811]* | 0.0128 | 0.46[0.238,0.899]* | 0.0256 | 0.32[0.133,0.773]* | 0.0140 |
| PSMB8 | 0.31[0.153,0.646]* | 0.0034 | 0.36[0.173,0.732]* | 0.0077 | 0.20[0.068,0.610]* | 0.0071 |
| PSMA5 | 0.91[0.433,1.913] | 0.7964 | 0.83[0.414,1.648] | 0.5668 | 0.84[0.239,2.959] | 0.7694 |
| DNMT1A | 0.51[0.210,1.235] | 0.1253 | 0.47[0.188,1.188] | 0.1042 | 0.58[0.192,1.748] | 0.3114 |
| DNMT3A | 0.69[0.355,1.357] | 0.270 | 0.73[0.373,1.425] | 0.3351 | 0.67[0.236,1.887] | 0.4166 |
| DNMT3B | 0.23[0.092,0.568]* | 0.0048 | 0.21[0.084,0.548]* | 0.0040 | 0.21[0.075,0.591]* | 0.0068 |
| HDAC1 | 0.52[0.366,0.741]* | 0.0009 | 0.60[0.425,0.851]* | 0.0065 | 0.43[0.243,0.754]* | 0.0058 |
| HDAC2 | 0.32[0.143,0.714]* | 0.0085 | 0.33[0.150,0.747]* | 0.0109 | 0.24[0.069,0.838]* | 0.0278 |
| HDAC4 | 0.66[0.328,1.317] | 0.2214 | 0.60[0.295,1.234] | 0.1554 | 0.74[0.256,2.120] | 0.5442 |
PBMC, peripheral blood mononuclear cells; CI, confidence interval; Relative expression ratio based on efficiency corrected delta delta Ct values presented as log values to the base 2, >1.5 indicates up regulation and ≤0.5 indicates down regulation in the experimental group compared to control group
* indicates statistically significant differential expression where P-value <0.05, P values calculated by paired t-tests