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Quantified protein expression of differentiated mESCs from immunostaining for (a) TuJ, (b) Nestin, and (c) GFAP. Each panel compares differentiation from a single mESC colony in co-culture with feeder PA6 cells in a microwell and each of nine isolated colonies of mESCs in co-culture with feeder PA6 cells in a Petri dish. *p<0.01. N=20.
