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Schematic representation of the experimental protocol. (A) Addition of stromal cells derived medium (SCM) from a Petri dish to a culture of a single colony of mESCs on PA6 cells in a well of 96-well plates moderately increases neural differentiation. (B) Addition of co-culture derived medium (CCM) from a Petri dish to a culture of a single colony of mESCs on PA6 cells in a well of 96-well plates Significantly enhances neural differentiation.
