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. 2017 Jul 5;3(7):e1700220. doi: 10.1126/sciadv.1700220

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Copyright © 2017 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

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Fig. 5 — (A and B) Representative negative stain images of MLAs formed in a reconstitution assay using a Cys-less PMP22 mutant (C42S, C53S, C85A, and C109A). Scale bars (A and B), 100 nm. (C) Quantification of the percentage of MLAs present in a series of negative stain EM images of WT and Cys-less PMP22 reconstitutions. All individual object counts were converted to the percentage of total counts for a particular sample and were normalized to the percentage of total counts represented by MLAs in the WT PMP22 control, which was set to 1.0. Green, WT control; red, Cys-less PMP22.