Fig. 3.

Localization of BC200 RNA and colocalization with hnRNP E2 in HeLa cells. (A) HeLa cells were treated with MabBC200-A3 and incubated with Cy™2 AffiniPure Donkey Anti-Human IgG, and the nuclei were stained with Hoechst 33342. BC200 RNA and the nuclei were visualized as green and blue fluorescence, respectively, under confocal microscopy. (B) HeLa cells were transfected with an hnRNP E2-eCFP-expressing construct, treated with MabBC200-A3, and incubated with Cy™2 AffiniPure Donkey Anti-Human IgG. BC200 RNA and hnRNP E2-eCFP were visualized as green and blue fluorescence, respectively, under confocal microscopy. Co-localization is shown by arrows.