Fig. 4.

Effects of chlorogenic acid (CGA) and store-operated channel (SOC) blockers on lysophosphatidylcholine (LPC)-induced ROS generation in human umbilical vein endothelial cells (HUVECs). (A) LPC increased ROS generation. CGA or SOC blockers decreased the LPC-induced ROS generation with a similar potency to N-acetyl-L-cysteine (NAC). The bar graph shows that CGA decreased the LPC-induced ROS generation with the same efficiency as a ROS generation inhibitor or SOC blockers gadolinium (Gd³⁺) and lanthanum (La³⁺) (B) Effects of ROS generation inhibitors on LPC-induced ROS generation in HUVECs. Rotenone inhibited LPC-induced ROS generation. The bar graph shows that rotenone decreased LPC-induced ROS generation to a greater extent than diphenyleneiodonium (DPI). Data are the mean ± SEM of three separate experiments. **P < 0.01 compared with a corresponding single treatment with the vehicle control; ^#P < 0.05, ^##P < 0.01 compared with a corresponding single treatment with 50 μmol/L LPC.