Table 1.

Comparison of the directed differentiation approaches for generating PGCLCs in aggregates. In all medias, PGCLCs are induced in the aggregates and isolated by FACS. Only the critical media additives are included. See text for details

PGCLC method	hESC/hiPSC self-renewal media	hESC/hiPSC self- renewal support/substrate	Pre-aggregate differentiation step	Aggregates containing PGCLCs
Irie (Irie et al., 2015)	LIF, bFGF, TGFβ1 CHIR99021 PD0325901 SB203580 SP600125	Irradiated MEFs	Not necessary	BMP4 LIF SCF EGF ROCK inhibitor
Sasaki (Sasaki et al., 2015)	StemFIT Commercial media	Laminin511	CHIR99021 ACTIVIN A on Fibronectin for 42 hours	BMP4 LIF SCF EGF ROCK inhibitor
Sugawa (Sugawa et al., 2015)	Conventional MEF conditioned media containing bFGF/KSR	Matrigel	ACTIVIN A, BMP4, bFGF, ROCK inhibitor on Matrigel for 48 hours	BMP4 LIF ROCK inhibitor