Figure 6.

Identification of ASOs that Provide Highly Effective Silencing of Mouse Adam33 Expression in Mouse Embryonic Fibroblasts

Oligonucleotides were delivered at 50 nM with Lipofectamine RNAiMAX. Error bars represent the SD of biological replicates. Mouse-targeted sequences are shown in Table 1 and were made as fully phosphorothioate (PS) 3-9-3 LNA gapmers (the same chemical architecture as the human sequences). The sequence of LNA-scr2, also a PS 3-9-3 gapmer, is 5′-AACacgtctataCGC-3′. 33-O is the human sequence from Figure 3, included as an additional negative control.