Table 1.
Subcellular distribution of the various published mammalian obscurin antibodies
| Epitope no.a | Antibody nameb | Subcellular localization | Reference |
|---|---|---|---|
| 1 | 5H10 | M-band (C/S) | Kontrogianni-Konstantopoulos et al. 2006 |
| 2 | 4A8 | M-band (C/S) | Kontrogianni-Konstantopoulos et al. 2006 |
| 3 | F6/I7 | M-band (C/S); Z-disc (C/S) | Bang et al. 2001 |
| 4 | Ob19-20 | M-band (C)c | Young et al. 2001 |
| 5 | Ob48-49 | M-band (Cc | Young et al. 2001 |
| 6 | I48/I49/F50 | Z-disc (C)c | Bang et al. 2001 |
| 7 | Ob51-52 | M-band (C)c | Young et al. 2001 |
| 8 | ObDH | M-band (C)c | Young et al. 2001 |
| 9 | RhoGEF | M-band (C/S) | Bowman et al. 2007 |
| 10 | ObscCOOH | M-band (C/S); Z-disc (C/S) | Kontrogianni-Konstantopoulos et al. 2003 |
| 11 | Ob6215-6353 | M-band (S)c | Bagnato et al. 2003 |
| 12 | ABD | M-band (C/S); Z-disc (C/S) | Ackermann et al. 2014 |
| 13 | SK2 | Nuclei (C); Z-disc (C)c | Borisov et al. 2008 |
| 14 | ObsKin-1 | M-band (C); Z-disc (C); ID (C)c | Hu and Kontrogianni-Konstantopoulos 2013 |
| 15 | ObsKin-2 | M-band (C); Z-disc (C); ID (C)c | Hu and Kontrogianni-Konstantopoulos 2013 |
| 16 | Link7 | Nuclei (C); Z-disc (C)c | Borisov et al. 2008 |
| 17 | ObscKin-3 | M-band (C); Z-disc (C); ID (C)c | Hu and Kontrogianni-Konstantopoulos 2013 |
ABD Ankyrin-binding domain; ID, intercalated disc; Ob, obscurin; RhoGEF, rho-guanine nucleotide exchange factor; SK2, one of two active Ser/Thr kinase motifs in the COOH-terminus of obscurin-B
aEpitope number corresponds to the epitope map found in Fig. 3
bAntibodies along the length of giant obscurins map to distinct epitopes of the proteins
cThose antibodies which have only been tested in either cardiac (C) OR skeletal (S) muscle, as indicated. All others in column (without superscript C or S) have been tested in both types of muscle