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. 2017 May 18;8(7):514–526. doi: 10.1007/s13238-017-0422-6

Figure 4.

Figure 4

T cells with a less differentiated phenotype and potent lytic activity were expanded by RNA co-electroporation. (A) Detection of less differentiated T cell markers using flow cytometry for T cells generated by OKT3/IL-2 (OKT3), OKT3-28BBZ RNA electroporation (OKT-28bb-1) or OKT3-28BBZ, CD86 and 4-1BBL RNA co-electroporation (OKT-28bb-2). (B) Four-hour flow-based CTL assay of CD19.BBZ CAR RNA-electroporated T cells