Table 2.
Phenotypic and acid tolerance characteristics of the isolates
| Soil sample code | Isolate code | Colony morphology | Growth at | Gram reactiona | Shape | Motility | Endospore | |||
|---|---|---|---|---|---|---|---|---|---|---|
| pH 4.5 | pH 4.0 | pH 3.5 | pH 4.5–acetate buffer | |||||||
| S1 | G1 | Light yellow, smooth, glistening, opaque, circular, convex with entire mergin | + | + | − | − | + | C | – | – |
| G2 | Cream white, opaque, and rhizoid | + | + | − | − | + | B | – | + | |
| G3 | White, circular, smooth, opaque | + | + | − | − | + | C | – | – | |
| G4 | Light orange, circular, slightly convex, smooth | + | + | − | − | + | C | – | – | |
| G5 | White, translucent, irregular with a spreading margin | + | + | − | − | − | B | + | – | |
| S2 | G6 | Orange, circular, slightly convex, smooth | + | + | − | − | + | C | – | – |
| G7 | Cream white, round, smooth, slightly mucoid with entire margins | + | + | − | + | + | B | + | + | |
| G8 | White, opaque, smooth, spreaded | + | + | − | + | + | B | + | + | |
| G9 | Dull white, rough, opaque, round | + | + | − | + | + | B | + | + | |
| G10 | Cream white, irregular, rough opaque | + | + | − | − | + | B | + | + | |
| S3 | G11 | Gray white, smooth opaque, irregular | + | − | − | − | + | B | + | + |
| G12 | Dull white, rough, opaque, irregular | + | + | − | − | + | B | + | + | |
| G13 | Yellowish, smooth opaque, irregular | + | + | − | − | + | B | + | + | |
| G14 | Yellowish, smooth opaque, irregular | + | + | − | − | + | B | + | + | |
| G15 | White, opaque, smooth, spreaded | + | + | − | − | + | B | + | + | |
| S4 | G16 | Orange, circular, slightly convex, smooth | + | + | − | − | + | C | – | – |
| G17 | Cream white, irregular, rough opaque | + | + | − | − | + | B | + | + | |
| G18 | Cream white, round, smooth, slightly mucoid with entire margins | + | + | − | + | + | B | + | + | |
| G19 | Cream, rough, opaque, irregular | + | + | − | − | + | B | + | + | |
| G20 | White, smooth, glistening, opaque, circular, convex with entire margin | + | − | − | − | − | B | + | – | |
| S5 | G21 | Cream, circular with undulate margin | + | + | − | + | + | B | + | + |
| G22 | White, rough, irregular, opaque | + | + | − | − | + | B | + | + | |
| G23 | Orange, circular, slightly convex, smooth | + | + | – | – | + | C | – | – | |
| G24 | Cream, irregular with undulate margin | + | + | – | – | + | B | + | + | |
| G25 | Light yellow, irregular with undulate margin | + | + | – | – | + | B | + | + | |
| S6 | G26 | White, circular with undulate margin | + | + | – | – | + | B | + | + |
| G27 | Dull white, rough, opaque, irregular | + | + | – | – | + | B | + | + | |
| G28 | White, smooth moist, irregular, opaque | + | + | – | + | + | B | + | + | |
| G29 | Cream white, opaque, and rhizoid | + | + | – | – | + | B | + | + | |
| G30 | Orange, circular, slightly convex, smooth | + | + | – | – | + | C | – | – | |
| S7 | G31 | White, smooth, translucent, flat round | + | + | – | – | + | B | + | + |
| G32 | White, rough, irregular, opaque | + | + | – | – | + | B | + | + | |
| G33 | Dull white, rough, opaque, irregular | + | + | – | – | + | B | + | + | |
| G34 | Light orange, rough, opaque, irregular | + | + | – | – | + | B | + | + | |
| G35 | White, round, opaque, smooth, spreaded | + | + | – | – | + | B | + | + | |
| S8 | G36 | White, round, flat with irregular edges | + | – | – | – | – | B | + | – |
| G37 | Cream white, irregular, rough opaque colonies | + | + | – | – | + | B | + | + | |
| G38 | Yellowish, smooth opaque, irregular, waxy | + | + | – | – | + | B | + | + | |
| G39 | White, opaque, smooth, spreaded | + | + | – | + | + | B | + | + | |
| G40 | White, translucent and irregular with a spreading margin | + | + | – | – | – | B | + | – | |
| S9 | G41 | White, opaque, smooth | + | + | – | – | + | B | + | + |
| G42 | White, round, flat with irregular edges | + | – | – | – | – | B | + | – | |
| G43 | White, undulate with irregular margins | + | + | – | + | + | B | + | + | |
| G44 | Yellowish, smooth opaque, irregular | + | + | – | + | + | B | + | + | |
| G45 | Dull white, rough, opaque, irregular | + | + | – | + | + | B | + | + | |
| S10 | G46 | Orange, circular, slightly convex, Smooth | + | + | – | + | + | C | – | – |
| G47 | White, sticky, Glistening moist colonies | + | – | – | – | – | B | – | – | |
| G48 | White, round, opaque, smooth | + | + | – | + | + | B | + | + | |
| G49 | Dull white, rough, opaque, irregular | + | + | – | – | + | B | + | + | |
| G50 | White, opaque, smooth, spreaded | + | + | – | – | + | B | + | + | |
| S11 | G51 | White, translucent and irregular with a spreading margin | + | – | – | – | – | B | + | – |
| G52 | White, smooth, opaque, circular, convex with entire edge | + | – | – | – | – | B | + | – | |
| G53 | Yellowish gray, circular, smooth | + | + | – | – | + | C | – | – | |
| G54 | Dull white, undulate, with irregular margins | + | + | – | – | + | B | + | + | |
| G55 | White, sticky, round with entire margin | + | + | – | – | – | B | + | – | |
| S12 | G56 | White, opaque, smooth, spreaded | + | + | – | – | + | B | + | + |
| G57 | Yellowish, rough, opaque, irregular | + | + | – | – | + | B | + | + | |
| G58 | Smooth, circular, convex, translucent | + | + | – | – | – | B | + | – | |
| G59 | Light yellow, circular, slightly convex, smooth | + | + | – | – | + | C | – | – | |
| G60 | Yellowish, smooth opaque, irregular | + | + | – | – | + | B | + | + | |
| S13 | G61 | White, smooth opaque, irregular | + | – | – | – | + | B | + | + |
| G62 | White, smooth opaque, irregular | + | – | – | – | + | B | + | + | |
| G63 | White, smooth opaque, round | + | – | – | – | + | B | + | + | |
| G64 | Yellowish, smooth opaque, irregular | + | – | – | – | + | B | + | + | |
| G65 | Yellowish, smooth mucoid opaque, round | + | – | – | – | + | B | + | + | |
| S14 | G66 | White, with a regular margin, convex | + | – | – | – | + | B | + | + |
| G67 | Yellowish, Smooth, glistening, opaque, circular with entire margin | + | – | – | – | – | B | + | – | |
| G68 | White, translucent, and irregular margin | + | + | – | – | – | B | + | – | |
| G69 | Smooth, circular, convex, translucent | + | + | – | – | – | B | + | – | |
| G70 | White, flat with a irregular margin | + | – | – | – | + | B | + | + | |
a B Bacillus/rod, C Coccus; + positive test, − negative test