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The experimental protocol. Controlled cortical impact (CCI) animals were treated with tauroursodeoxycholic acid (TUDCA), saline, MK2206 or Akt siRNA. TUDCA, saline or MK2206 were treated for 3 days following traumatic brain injury (TBI), whereas Akt siRNA were treated for once. The time points at which we chose the samples from each group for western blotting, immunofluorescence, TUNEL staining and RT-PCR are also indicated.
