Table 1.
Antibodies for western blot.
| Antibody | Catalog # | Vendor | Dilution | Molecular weight (kDa) |
|---|---|---|---|---|
| GRP78 | NBP1-06274 | Novusbio | 1:250 | 75 |
| p-PERK (Thr981) | SC-32577 | Santa | 1:500 | 170 |
| PERK | 3192 | CST | 1:1000 | 140 |
| p-eIF2a (Ser51) | Ab32157 | CST | 1:1000 | 36 |
| eIF2a | 9722 | CST | 1:1000 | 38 |
| ATF4 | 11815 | CST | 1:1000 | 49 |
| p-Akt (Ser473) | 4060 | CST | 1:1000 | 60 |
| Akt | 9272 | CST | 1:1000 | 60 |
| Pten | 9552 | CST | 1:1000 | 54 |
| CHOP | 2895 | CST | 1:1000 | 27 |
| Caspase-12 | AB62484 | Abcam | 1:1000 | 42 |
| Bax | 2722 | CST | 1:1000 | 20 |
| Bcl-2 | 2876 | CST | 1:1000 | 28 |
| β-Actin | 3700 | CST | 1:1000 | 45 |
| Peroxidase-Conjugated anti-Rabbit IgG (H + L) | ZB-2305 | ZSGB-BIO | 1:5000 | |
| Peroxidase-Conjugated anti-Mouse IgG (H + L) | ZB-2301 | ZSGB-BIO | 1:5000 |