Figure 5.

Endocytosis of pRNA is needed for triggering bactericidal responses. Isolated PMN were incubated with pRNA in the presence or absence of the endocytosis inhibitor, Nystatin (Nys). Results were expressed as the mean ± ES, n = 5. (A) Percentage of FSC high PMN. Right panel: Representative dot-plots showing the SSC vs. FSC profiles of PMN incubated with pRNA or pRNA+Nys. (B) Expression of CD11b. Results were expressed as the relative fluorescence (RF) of the MFI of the different treatments with respect to untreated PMN. Right panel: Representative histogram showing the CD11b expression of PMN left untreated or incubated with pRNA, pRNA+Nys, and Nys. (C) ROS generation. Right panel: Representative dot-plots of the percentage of DHR+ PMN are shown in untreated (−) or incubated with pRNA, pRNA+Nys, and Nys. (D) NETs formation. Right panel: Representative microphotographs of confocal images showing PMN from pRNA and pRNA+Nys groups stained for DNA or elastase visualization and the merge of the images (x200). In all cases ^*p < 0.05 vs. all other groups and ^#p < 0.05 vs. pRNA.